Hot off the presses! Sep 01 Nat Rev Mol Cell Biol

The Sep 01 issue of the Nat Rev Mol Cell Biol is now up on Pubget (About Nat Rev Mol Cell Biol): if you're at a subscribing institution, just click the link in the latest link at the home page. (Note you'll only be able to get all the PDFs in the issue if your institution subscribes to Pubget.)

Latest Articles Include:

• From the editors
  - Nat Rev Mol Cell Biol 10(9):575 (2009)
  
• Proteomics: Getting the numbers right
  - Nat Rev Mol Cell Biol 10(9):577 (2009)
  
• Stem cells: The great iPS barrier
  - Nat Rev Mol Cell Biol 10(9):578 (2009)
  
• Small RNAs: Bound to silence
  - Nat Rev Mol Cell Biol 10(9):578 (2009)
  
• In brief: Endocytosis, Endocytosis, Endocytosis
  - Nat Rev Mol Cell Biol 10(9):579 (2009)
  
• Small RNAs: p53 makes microRNAs mature
  - Nat Rev Mol Cell Biol 10(9):580 (2009)
  
• Cytoskeleton: Getting to the TIP
  - Nat Rev Mol Cell Biol 10(9):580 (2009)
  
• In brief: RNA decay, Autophagy, RNA interference
  - Nat Rev Mol Cell Biol 10(9):580 (2009)
  
• Endocytosis: Joint efforts of ESCRTs
  - Nat Rev Mol Cell Biol 10(9):581 (2009)
  
• Seeing is believing
  - Nat Rev Mol Cell Biol 10(9):582 (2009)
  
• Chromatin: A positive twist
  - Nat Rev Mol Cell Biol 10(9):582 (2009)
  
• Tickets to ride: selecting cargo for clathrin-regulated internalization
  - Nat Rev Mol Cell Biol 10(9):583-596 (2009)
  Clathrin-mediated endocytosis oversees the constitutive packaging of selected membrane cargoes into transport vesicles that fuse with early endosomes. The process is responsive to activation of signalling receptors and ion channels, promptly clearing post-translationally tagged forms of cargo off the plasma membrane. To accommodate the diverse array of transmembrane proteins that are variably gathered into forming vesicles, a dedicated sorting machinery cooperates to ensure that non-competitive uptake from the cell surface occurs within minutes. Recent structural and functional data reveal remarkable plasticity in how disparate sorting signals are recognized by cargo-selective clathrin adaptors, such as AP-2. Cargo loading also seems to govern whether coats ultimately bud or dismantle abortively at the cell surface.
• Pathways and mechanisms of endocytic recycling
  - Nat Rev Mol Cell Biol 10(9):597-608 (2009)
  Endocytic recycling is coordinated with endocytic uptake to control the composition of the plasma membrane. Although much of our understanding of endocytic recycling has come from studies on the transferrin receptor, a protein internalized through clathrin-dependent endocytosis, increased interest in clathrin-independent endocytosis has led to the discovery of new endocytic recycling systems. Recent insights into the regulatory mechanisms that control endocytic recycling have focused on recycling through tubular carriers and the return to the cell surface of cargoes that enter cells through clathrin-independent mechanisms. Recent work emphasizes the importance of regulated recycling in processes as diverse as cytokinesis, cell adhesion, morphogenesis, cell fusion, learning and memory.
• Endocytosis and signalling: intertwining molecular networks
  - Nat Rev Mol Cell Biol 10(9):609-622 (2009)
  Cell signalling and endocytic membrane trafficking have traditionally been viewed as distinct processes. Although our present understanding is incomplete and there are still great controversies, it is now recognized that these processes are intimately and bidirectionally linked in animal cells. Indeed, many recent examples illustrate how endocytosis regulates receptor signalling (including signalling from receptor tyrosine kinases and G protein-coupled receptors) and, conversely, how signalling regulates the endocytic pathway. The mechanistic and functional principles that underlie the relationship between signalling and endocytosis in cell biology are becoming increasingly evident across many systems.
• Lysosome biogenesis and lysosomal membrane proteins: trafficking meets function
  - Nat Rev Mol Cell Biol 10(9):623-635 (2009)
  Lysosomes are the primary catabolic compartments of eukaryotic cells. They degrade extracellular material that has been internalized by endocytosis and intracellular components that have been sequestered by autophagy. In addition, specialized cells contain lysosome-related organelles that store and secrete proteins for cell-type-specific functions. The functioning of a healthy cell is dependent on the proper targeting of newly synthesized lysosomal proteins. Accumulating evidence suggests that there are multiple lysosomal delivery pathways that together allow the regulated and sequential deposition of lysosomal components. The importance of lysosomal trafficking pathways is emphasized by recent findings that reveal new roles for lysosomal membrane proteins in cellular physiology and in an increasing number of diseases that are characterized by defects in lysosome biogenesis.
• Regulatory roles of natural antisense transcripts
  Faghihi MA Wahlestedt C - Nat Rev Mol Cell Biol 10(9):637-643 (2009)
  Mammalian genomes encode numerous natural antisense transcripts, but the function of these transcripts is not well understood. Functional validation studies indicate that antisense transcripts are not a uniform group of regulatory RNAs but instead belong to multiple categories with some common features. Recent evidence indicates that antisense transcripts are frequently functional and use diverse transcriptional and post-transcriptional gene regulatory mechanisms to carry out a wide variety of biological roles.
• Biology under construction: in vitro reconstitution of cellular function
  - Nat Rev Mol Cell Biol 10(9):644-650 (2009)
  We are much better at taking cells apart than putting them together. Reconstitution of biological processes from component molecules has been a powerful but difficult approach to studying functional organization in biology. Recently, the convergence of biochemical and cell biological advances with new experimental and computational tools is providing the opportunity to reconstitute increasingly complex processes. We predict that this bottom-up strategy will uncover basic processes that guide cellular assembly, advancing both basic and applied sciences.