Hot off the presses! May 01 Nature Reviews Molecular Cell Biology

The May 01 issue of the Nature Reviews Molecular Cell Biology is now up on Pubget (About Nature Reviews Molecular Cell Biology): if you're at a subscribing institution, just click the link in the latest link at the home page. (Note you'll only be able to get all the PDFs in the issue if your institution subscribes to Pubget.)

Latest Articles Include:

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  - Nature Reviews Molecular Cell Biology 11(5):309 (2010)
  
• Cell cycle: Dissecting mitosis | PDF (334 KB)
  - Nature Reviews Molecular Cell Biology 11(5):310 (2010)
  Two high-throughput studies identify genes and proteins involved in mitosis.
• Mechanotransduction: Using the force | PDF (187 KB)
  - Nature Reviews Molecular Cell Biology 11(5):311 (2010)
  Chemo-mechanical coupling in EPHA2â€"ephrin A1 signalling.
• Masters of the genome | PDF (179 KB)
  - Nature Reviews Molecular Cell Biology 11(5):311 (2010)
  The path to the discovery of transcriptional co-activators.
• Protein folding: Dual chaperone function | PDF (177 KB)
  - Nature Reviews Molecular Cell Biology 11(5):312 (2010)
  Chaperones function in protein folding and ribosome biogenesis.
• Development: Getting to the heart of the matter | PDF (356 KB)
  - Nature Reviews Molecular Cell Biology 11(5):312 (2010)
  Coronary vessels derive from endothelial cells of the sinus venosus.
• Development: Going with the flow | PDF (297 KB)
  - Nature Reviews Molecular Cell Biology 11(5):313 (2010)
  Planar cell polarity regulates the positioning and beating of cilia.
• Metabolic disease: Muscular fat burning | PDF (238 KB)
  - Nature Reviews Molecular Cell Biology 11(5):314 (2010)
  VEGFB as a new regulator of fatty acid uptake.
• Endocytosis: Division of labour between ESCRTs | PDF (197 KB)
  - Nature Reviews Molecular Cell Biology 11(5):314 (2010)
  This study reveals the role of each ESCRT complex in MVB biogenesis.
• Signal peptide recognition | Cell–cell interactions | PDF (166 KB)
  - Nature Reviews Molecular Cell Biology 11(5):314 (2010)
  Signal peptide recognition Most secretory and membrane proteins contain a signal peptide, which is recognized by the signal recognition particle (SRP) when newly synthesized polypeptide chains emerge from the ribosome. The signal peptide targets the SRPâ€"ribosome nascent chain (RNC) complex to protein-translocating channels in the endoplasmic reticulum membrane in eukaryotes and in the plasma membrane in bacteria.
• Phagocytosis | Stem cells | Gene expression | PDF (166 KB)
  - Nature Reviews Molecular Cell Biology 11(5):315 (2010)
  Phagocytosis Identification of two evolutionarily conserved genes regulating processing of engulfed apoptotic cells Kinchen, J. M. & Ravichandran, K. S. Nature 464, 778â€"782 (2010) Apoptotic cells are engulfed by phagosomes and degraded by phagosome maturation. RAB-5 and RAB-7 are sequentially recruited to phagosomes that contain apoptotic cells, and SAND-1 (the worm homologue of mammalian MON1A) has been proposed to function upstream of RAB-7 in the phagosome maturation process.
• The nuclear envelope in genome organization, expression and stability
  Mekhail K Moazed D - Nature Reviews Molecular Cell Biology 11(5):317 (2010)
  Non-random positioning of chromosomal domains relative to each other and to nuclear landmarks is a common feature of eukaryotic genomes. In particular, the distribution of DNA loci relative to the nuclear periphery has been linked to both transcriptional activation and repression. Nuclear pores and other integral membrane protein complexes are key players in the dynamic organization of the genome in the nucleus, and recent advances in our understanding of the molecular networks that organize genomes at the nuclear periphery point to a further role for non-random locus positioning in DNA repair, recombination and stability.
• The emerging mechanisms of isoform-specific PI3K signalling
  Vanhaesebroeck B Guillermet-Guibert J Graupera M Bilanges B - Nature Reviews Molecular Cell Biology 11(5):329 (2010)
  Phosphoinositide 3-kinases (PI3Ks) function early in intracellular signal transduction pathways and affect many biological functions. A further level of complexity derives from the existence of eight PI3K isoforms, which are divided into class I, class II and class III PI3Ks. PI3K signalling has been implicated in metabolic control, immunity, angiogenesis and cardiovascular homeostasis, and is one of the most frequently deregulated pathways in cancer. PI3K inhibitors have recently entered clinical trials in oncology. A better understanding of how the different PI3K isoforms are regulated and control signalling could uncover their roles in pathology and reveal in which disease contexts their blockade could be most beneficial.
• Spatial organization and signal transduction at intercellular junctions
  Manz BN Groves JT - Nature Reviews Molecular Cell Biology 11(5):342 (2010)
  The coordinated organization of cell membrane receptors into diverse micrometre-scale spatial patterns is emerging as an important theme of intercellular signalling, as exemplified by immunological synapses. Key characteristics of these patterns are that they transcend direct protein–protein interactions, emerge transiently and modulate signal transduction. Such cooperativity over multiple length scales presents new and intriguing challenges for the study and ultimate understanding of cellular signalling. As a result, new experimental strategies have emerged to manipulate the spatial organization of molecules inside living cells. The resulting spatial mutations yield insights into the interweaving of the spatial, mechanical and chemical aspects of intercellular signalling.
• Linking actin dynamics and gene transcription to drive cellular motile functions
  Olson EN Nordheim A - Nature Reviews Molecular Cell Biology 11(5):353 (2010)
  Numerous physiological and pathological stimuli promote the rearrangement of the actin cytoskeleton, thereby modulating cellular motile functions. Although it seems intuitively obvious that cell motility requires coordinated protein biosynthesis, until recently the linkage between cytoskeletal actin dynamics and correlated gene activities remained unknown. This knowledge gap was filled in part by the discovery that globular actin polymerization liberates myocardin-related transcription factor (MRTF) cofactors, thereby inducing the nuclear transcription factor serum response factor (SRF) to modulate the expression of genes encoding structural and regulatory effectors of actin dynamics. This insight stimulated research to better understand the actin–MRTF–SRF circuit and to identify alternative mechanisms that link cytoskeletal dynamics and genome activity.
• Breaching multiple barriers: leukocyte motility through venular walls and the interstitium
  Nourshargh S Hordijk PL Sixt M - Nature Reviews Molecular Cell Biology 11(5):366 (2010)
  The shuttling of leukocytes between the bloodstream and interstitial tissues involves different locomotion strategies that are governed by locally presented soluble and cell-bound signals. Recent studies have furthered our understanding of the rapidly advancing field of leukocyte migration, particularly regarding cellular and subcellular events at the level of the venular wall. Furthermore, emerging cellular models are now addressing the transition from an adherent mode to a non-adherent state, incorporating mechanisms that support an efficient migratory profile of leukocytes in the interstitial tissue beyond the venular wall.
• Role of GW182 proteins and PABPC1 in the miRNA pathway: a sense of déjà vu
  Tritschler F Huntzinger E Izaurralde E - Nature Reviews Molecular Cell Biology 11(5):379 (2010)
  GW182 proteins have emerged as key components of microRNA (miRNA) silencing complexes in animals. Although the precise molecular function of GW182 proteins is not fully understood, new findings indicate that they act as poly(A)-binding protein (PABP)-interacting proteins (PAIPs) that promote gene silencing, at least in part, by interfering with cytoplasmic PABP1 (PABPC1) function during translation and mRNA stabilization. This recent discovery paves the way for future studies of miRNA silencing mechanisms.